Nongenetic heterogeneity of mouse beta-galactosidase.
نویسندگان
چکیده
منابع مشابه
Delivery of beta-galactosidase to mouse brain via the blood-brain barrier transferrin receptor.
Enzyme replacement therapy of lysosomal storage disorders is complicated by the lack of enzyme transport across the blood-brain barrier (BBB). The present studies evaluate the delivery of a model enzyme across the BBB following enzyme conjugation to a BBB receptor-specific monoclonal antibody (mAb). Bacterial beta-galactosidase (116 kDa) was conjugated to the rat 8D3 mAb to the rat transferrin ...
متن کاملEffect of swainsonine on the processing and turnover of lysosomal beta-galactosidase and beta-glucuronidase from mouse peritoneal macrophages.
The effect of swainsonine, an inhibitor of Golgi alpha-mannosidase II and lysosomal alpha-mannosidase, on the synthesis, processing, and turnover of two glycoproteins, lysosomal beta-galactosidase and lysosomal beta-glucuronidase, has been studied in cultured mouse peritoneal macrophages. No effect of the inhibitor on the relative rates of synthesis of the precursor form of either enzyme was ob...
متن کاملDevelopment of a Nongenetic Mouse Model of Type 2 Diabetes
Insulin resistance and loss of β-cell mass cause Type 2 diabetes (T2D). The objective of this study was to generate a nongenetic mouse model of T2D. Ninety-six 6-month-old C57BL/6N males were assigned to 1 of 12 groups including (1) low-fat diet (LFD; low-fat control; LFC), (2) LFD with 1 i.p. 40 mg/kg BW streptozotocin (STZ) injection, (3), (4), (5), (6) LFD with 2, 3, 4, or 5 STZ injections o...
متن کاملBioinformatics Designing of 10-23 Deoxyribozyme against Coding Region of Beta-galactosidase Gene
Background: Deoxyribozymes (Dzs) can play a role as gene expression inhibitors at mRNA level. Among Dzs, the 10-23 deoxyribozyme has significant potentials for treatment of diseases. Designed Dz includes a catalytic core made of 15 deoxyribonucleotides and two binding arms consisted of 6-12 nucleotides for site specific binding to target RNA and hydrolysis. The enzyme has characteristic feature...
متن کاملAnalysis of the differentiation and of the heterogeneity within a population of Escherichia coli undergoing induced beta-galactosidase synthesis.
The kinetics of induction of the galactozymase of certain yeasts (Spiegelman, 1951) and the permease-3-galactosidase system of Escherichia coli (Monod, 1956) can, under certain conditions, be described as autocatalytic. Essentially two hypotheses have been proposed to account for such findings: (a) that the enzyme-forming system itself is autocatalytically activated (Campbell and Spiegelman, 19...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1977
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)40206-7